Fungi are everywhere around us and recently, there has been an increased interest in their role and importance in the forest. The fungi have several strategies to acquire energy and nutrition; one example is a mutual symbiosis with trees and plants - another being dissolving organic materials. Attempts have been made to better understand the circulation of nutrition in coniferous forests, and how fungi cooperate. Trees and plants have adapted with mycorrhizae and are now interdependent which is important to consider in forestry. A great number of fungi depend on dead wood and old trees to set fruiting bodies and spread further. These fungi also create environments for other species, such as birds and insects. The vegetation of a forest is determined by a series of factors; tree species, how old the forest is, soil type, as well as the ability of the fungal spores to spread and establish. Furthermore, in determining vegetation, the fungi?s ability to compete in the soil matters. These factors are nearly unexplored. Researching fungi knowledge and interest, a survey was distributed to five groups of people. The groups included forest owners, high school teachers, high school students, senior citizens, and a fifth group - ?o he ? - consisting of people of mixed ages and backgrounds.

Understory vegetation and fungi are regarded as important ecological drivers of processes like productivity and nutrient cycling in boreal forests. Whilst those processes are linked to soil water content, relatively little is known about the sources of soil water for these forest components. During early autumn in boreal forests, temperature falls and large events of rain are frequent which may influence soil water availability. To better understand the autumn plant-soil-fungi water relationships in this ecosystem, I used stable isotopes techniques in this study to examine the water sources for ericaceous shrubs and fungi in a Scots pine forest following a large, early autumn rain event. I hypothesize that ericaceous shrubs of two functional groups (evergreen vs.

The bean bruchid, Acanthoscelides obtectus is a major threat to bean production. In Ethiopia, where this study was performed, the damage by bruchids on stored beans has been reported to reach up to 38%. To use the isolates Beauveria bassiana and Metarhizium anisopliae in the management of A. obtectus can be an environmentally safe alternative for controlling the pest. In the present work the aim was to examine if the four chosen fungal isolates, B.

Broad beans are becoming a larger crop in Sweden. This is probably due to the current discussion about the cultivation of soya beans that are imported to be used as protein fodder. Broad beans are a good break crop in a small grain intensive crop rotation and works as a protein source for animals together with for example ley. At present little research has been done on broad beans and more knowledge is needed. This study is about fungal communities and diseases on broad beans and the impact of these fungi on seed germination.

The fallout from the Chernobyl nuclear accident resulted in large deposits of caesium, iodineand strontium as well as noble gases in various parts of Sweden. 137Cs has a radioactive halflifeof about 30 years and is therefore one of few radio-fission products remaining inmeasurable quantities in the Swedish soil. Radiocaesium behaves similarly to potassium insoil-plant systems and is easily absorbed by plants. It is important to understand how 137Csbehaves in different ecosystems in order to minimize the risk toward humans.The aim of this study was to determine how potassium fertilization influences radiocaesiumcontent in different forest plants and fungi. Potassium fertilizer was spread once in 1992 withapproximately 200 kg KCl per ha.

The Lodgepole pines susceptibility to Heterobasidion spp. was investigated in three parts ofthis study. In the first part, discs from freshly cut stumps were collected and analyzed to seeif the fungi can spread and grow naturally in Lodgepole pine stands. In the second part, rootsamples were collected from Lodgepole pines planted in former infected Norway sprucestands to see if the fungi can spread through root contacts. For the third part, new stumpswas created and artificially infected with spores of both the S?type (H.

Urban trees have very little in common with trees on natural sites, such as forests or pastures. Trees in urban environments are often planted in packed soil and in small volumes. This can lead to decreased availability of water and oxygen, as well as deteriorated ability for the soil to store nutritional elements. Also above ground the tree usually have limited space in cities. Regulations on free height over roads and cycle tracks demands high stems at an early stage of their development.

Identification of fungi is based on macroscopic observations of morphology and microscopic characteristics. These conventional methods are time-consuming and requires expert knowledge. For the past years Matrix-assisted laser desorption ionization-time of flight mass spectrometry has been used for routine bacterial identification in clinical laboratories but not yet in the same extension for fungi. In this study three preanalytic preparation methods for fungi were evaluated in order to shorten the processing time in routine laboratory performance.Clinically relevant strains (n=18) of molds and dermatophytes were cultivated on agar plates and prepared according to the different preparation methods for protein extraction. Each strain was analyzed in quadruplicate by the MALDI Biotyper and the database Filamentous Fungi Library 1.0.The results showed that the genus and species identification rates of the least time-consuming direct extraction method were 33% and 11% respectively.

Oilseed rape is an important crop in Sweden and is grown on about 90000 ha today. The interest in oil seed pro-duction is increasing because of growing demand and high prices for oil seed both in Sweden and Europe. However, an increased intensity in the oil seed production can bring higher risk of yield losses due to fungal diseases. The purpose of this study was to investigate what different fungi are associated with oilseed rape under field conditions and if their incidence is correlated to crop establishment problems. This study was carried out in three parts: 1) investigations on fungal community structure in roots and rhizosphere soils from young spring- and winter rape plants sampled in failed respectively good establishment patches in four fields, 2) investigations on the pathogenicity of fungi isolated from oilseed rape in controlled environment and 3) investigations on the effect of different fungicide seed treatments on germination and early plant development in a field soil naturally infested with damping off- and Verticillium wilt pathogens. The studies on community structure using a combined approach of standard fungal isolation techniques and DNA based methods revealed a high fungal diversity in three out of four studied fields of spring rape and winter rape. For some unknown reason, very few fungal species were identified from the fourth field.

Fusarium head blight is a large problem world wide which reduces the yields and the quality of small grains. Shrunken kernels with decreased thousand kernel weight as an effect and pro-duction of mycotoxins are some of the problems caused by infection of Fusarium spp. To avoid toxin contaminated bulks to enter the food and feed chain it is important to have tools for prediction and analysis of the fungi and toxins. Good prediction methods are also impor-tant in the breeding industry since early identification in trials of lines with high resistance or susceptibility simplify the work. To compare different techniques for these objectives this project was initiated.

Fusarium head blight is a large problem world wide which reduces the yields and the quality of small grains. Shrunken kernels with decreased thousand kernel weight as an effect and pro-duction of mycotoxins are some of the problems caused by infection of Fusarium spp. To avoid toxin contaminated bulks to enter the food and feed chain it is important to have tools for prediction and analysis of the fungi and toxins. Good prediction methods are also impor-tant in the breeding industry since early identification in trials of lines with high resistance or susceptibility simplify the work. To compare different techniques for these objectives this project was initiated.

During the last ten years the cultivation of maize in Sweden has increased and is expected to grow further. Most of the maize in Sweden becomes silage which is used to feed animals at farms. Maize has in other countries been shown to be a substrate for growth of mould and especially Aspergillus spp., Fusarium spp. and Pencillium spp. has been reported.

It has been observed by both onion producers and a plant protection advisor on Öland (an island off the east coast of Sweden) that basal rot is the largest contributory factor to reduced onion quality and yield. Basal rot is mainly caused by species of Fusarium fungi. The aim of this study was to: a) investigate which species of Fusarium that can be found in onion produced on Öland, b) describe the symptoms caused by the different Fusarium fungi found and c) explore, through interviews with the onion producers on Öland, the mechanisms that may be involved in the observed increase in basal rot.Onion bulbs (Allium cepa) were sampled on two occasions. In total 181 onions from 11 different fields were analysed. In addition, eight onion producers were interviewed.

The effect of inoculation on containerized pine and spruce seedlings in a nursery with two ectomycorrhizal fungi was investigated in regard to the development of mycorrhiza and plant growth. The fungi were Elaphomyces muricatus and Elaphomyces granulates and the inoculated seedlings were compared to an uninoculated control. These seedlings were all fertilized with arginine. Five mycorrhiza species were found after DNA sequencing of the mycorrhizal root tips with different found morphotypes on seedlings from all treatments. Thelephora terrestris was the type which had the greatest distribution on pine seedlings and Tylospora asterophora was found at big extent on spruce seedlings. The inoculated species was not found, if it was due to the species hadn?t colonized the roots or if the DNA sequencing failed we can only speculate about.

Allanblackia is a tree genus native to humid forests in West, Central and East Africa. Its fruits contain large seeds from which edible high-quality fat can be extracted. In order to create a sustainable supply chain of Allanblackia seeds, a domestication program has been initiated with the purpose to enable smallholder farmers to grow the tree as an agroforestry component and cash crop. More knowledge is required to understand the tree?s biology and possible symbioses involving soil microorganisms with plant growth enhancing poten-tial.